RNASeq library preparation
Contents
Total RNA
mRNA-seq workflow
Illumina NGS Library
Illumina library fragment
Illumina library read bindings 1
Illumina library read bindings 2
RNA-Seq applications
- rRNA depleted samples
- Very high dynamic range
- No prior knowledge of expressed genes.
- - Gives more information than microarrays.
- Applications
- - Sequencing of mRNA
- - Differential expression of known or unknown transcripts during a treatment or condition
- - Isoform detection.
- - Alternative splicing events
- - Post-transcriptional mutations or editing
- - Gene fusions
- - Non-coding RNAs
- - miRNAs/small non-Coding RNA
NGS Data Analysis
The number of replicates issue
