Difference between revisions of "SPAdes"

From wiki
Jump to: navigation, search
Line 2: Line 2:
  
 
Pavel Pevszner's de-novo assembler, primarily for - but not restricted to - bacteria.
 
Pavel Pevszner's de-novo assembler, primarily for - but not restricted to - bacteria.
 +
 +
 +
= Usage =
 +
  
 
= Installation (Sysadmin notes)=
 
= Installation (Sysadmin notes)=
  
Initially version 3.7.0 was insatlled using the specially compiled gcc/4.9.3 compiler (available as a module). However the '''-b''' version of the module now uses Redhat''s devtoolset-2,
+
Initially version 3.7.0 was installed using the specially compiled gcc/4.9.3 compiler (available as a module). However the '''-b''' version of the module now uses Redhat''s devtoolset-2,
 
so that this compiler is not necessary.
 
so that this compiler is not necessary.
  
Line 24: Line 28:
  
 
For the truspades modality.
 
For the truspades modality.
 +
 +
= Links =
 +
* [http://spades.bioinf.spbau.ru/release3.8.1/manual.html Official manual]

Revision as of 13:44, 23 June 2016

Introduction

Pavel Pevszner's de-novo assembler, primarily for - but not restricted to - bacteria.


Usage

Installation (Sysadmin notes)

Initially version 3.7.0 was installed using the specially compiled gcc/4.9.3 compiler (available as a module). However the -b version of the module now uses Redhats devtoolset-2, so that this compiler is not necessary.

Boost however, is necessary. The cluster has the latest version: 1.60. Possibly compiled (well, the bits that can be compiled) with g++ 4.4.7. In any case, the location of boost is a problem, although the boost module on the cluster does create some useful environmental variables, the given stacks_compile script does recognise them.

In any case, the configure system is cmake, so a "build" subdirectory should be created. Inside that, a short compile script containined something the following should be created:

module load boost
cmake -G "Unix Makefiles" -DCMAKE_INSTALL_PREFIX=.. -DBoost_NO_BOOST_CMAKE=TRUE -DBoost_NO_SYSTEM_PATHS=TRUE -DBOOST_ROOT:PATHNAME=${BOOST_ROOT} -DBoost_INCLUDE_DIRS:FILEPATH=${BOOST_INCLUDEDIR} -DBoost_LIBRARY_DIRS:FILEPATH=${BOOST_LIBRARYDIR} ../src

There is no make test nor make check. What there is, however, is a test script in the installation (not the source) directory, whihc can be invoked as follows:

<spades installation dir>/spades.py --test

or

<spades installation dir>/truspades.py --test

For the truspades modality.

Links