This is a sister program to RepeatMasker. It also has no publication behind it.
- NB always use -engine ncbi when running RepeatModeler.
- NB2 There is a warning like so in the output:
Use of uninitialized value $1 in string ne at /shelf/modulefiles/tools/RepeatModeler/1.0.10/SequenceSimilarityMatrix.pm line 273, <MATRIX> line 7
Because it is only a warning, it should not affect the proper running of the program. In any case, it will be repaired shortly.
Here are the contents of the help manual:
$ RepeatModeler --help No database indicated NAME RepeatModeler - Model repetitive DNA SYNOPSIS RepeatModeler [-options] -database <XDF Database> DESCRIPTION The options are: -h(elp) Detailed help -database The prefix name of a XDF formatted sequence database containing the genomic sequence to use when building repeat models. The database may be created with the WUBlast "xdformat" utility or with the RepeatModeler wrapper script "BuildXDFDatabase". -engine <abblast|wublast|ncbi> The name of the search engine we are using. I.e abblast/wublast or ncbi (rmblast version). -pa # Specify the number of shared-memory processors available to this program. RepeatModeler will use the processors to run BLAST searches in parallel. i.e on a machine with 10 cores one might use 1 core for the script and 9 cores for the BLAST searches by running with "-pa 9". -recoverDir <Previous Output Directory> If a run fails in the middle of processing, it may be possible recover some results and continue where the previous run left off. Simply supply the output directory where the results of the failed run were saved and the program will attempt to recover and continue the run. -srand # Optionally set the seed of the random number generator to a known value before the batches are randomly selected ( using Fisher Yates Shuffling ). This is only useful if you need to reproduce the sample choice between runs. This should be an integer number. SEE ALSO RepeatMasker, WUBlast COPYRIGHT Copyright 2005-2017 Institute for Systems Biology AUTHOR Robert Hubley <firstname.lastname@example.org> Arian Smit <email@example.com>
Let's try Repeatmodeler on the S288C yeast reference genome. First the database must be built:
BuildDatabase -name yeast288c -engine ncbi S288_maniid.fsa
This is only a wrapper to the blast makedatabase function, and should finish quickly depending on he size of the file of course. Yeast is small (12MB), so it was over quickly.
RepeatModeler has a number of dependencies. How these are installed are detailed below:
nseg and nmerge (included in nseg) are quite old programs coded in C by NCBI. These were installed on all machines in /usr/local/bin
The same is done with RECON, which consists of the following executables:
So all these are now available in /usr/local/bin in the nodes as well.
As this also only includes two C-coded executables with quite good names, it is easy enough to install it locally on all the nodes:
and also the following perl scripts (using the unusual *.prl extension) must be also be installed:
This is quite similar to RepeatMasker, although there is an opportunity to manually edit RepModelConfig.pm.tmp which is more accurate and convenient than the automatic configure script. Predictably, it will only be active when the tmp extension is hived off.