Difference between revisions of "RNASeq library preparation"

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Revision as of 09:32, 9 May 2017

Total RNA

Totalrna.png

mRNA-seq workflow

Rnawf.png

Illumina NGS Library

Illu.png

Illumina library fragment

Illu2.png

Illumina library read bindings 1

Illu4.png

Illumina library read bindings 2

Illu5.png

RNA-Seq applications

  • rRNA depleted samples
  • Very high dynamic range
  • No prior knowledge of expressed genes.
- Gives more information than microarrays.
  • Applications
- Sequencing of mRNA
- Differential expression of known or unknown transcripts during a treatment or condition
- Isoform detection.
- Alternative splicing events
- Post-transcriptional mutations or editing
- Gene fusions
- Non-coding RNAs
- miRNAs/small non-Coding RNA

NGS Data Analysis

Dan.png