Intro to RNA-Seq Data Analysis Course

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Course schedule

  • This is based on a 2 day Edinburgh Genomics course of the same name.
  • Intro to Linux, Sequencer visits and technology iparts taken out. Also, less theory.
  • Maximise practical aspect.
  • Having said that, if you fall behind, listening is better than catching up

Course website: http://stab.st-andrews.ac.uk/i2rda/

Connecting to a remote Machine

Presenting this before introduction as some people might experience delays logging in.

  • We shall use a remote machine not the machine you are logged into locally
  • The program we shall use is PuTTY.
  • Please try to locate PuTTY in the applications section or on AppsAnywhere

Configuring PuTTY for connection

  • Server: marvin.st-andrews.ac.uk
  • Terminal | keyboard | check VT100+
  • Window | Selection | Control use of Mouse | set xterm
  • Connect | Data | enter username
  • Connection | ssh | X11 Forwarding | Check yes
  • Back to PuTTY main screen | select Default setting |click save

You should now be able to "open" a session

  • Be aware typng in your password is done blindly. I.e. it does not appear on the screen.

Overview of RNA-Seq

  • For gene expression analyses, seen as a more powerful replacememnt to microarrays

Computing resources

  • RNA-Seq is a heavy workload, we need to be prepared for long-running tasks. This has two implications
- The marvin cluster is an 11-machine shared computing resource, not a personal computer ... others are using it.
- We want to be able to have a process run unattended.
  • For these two aspects, we have:
  • A queue system to use, we shall request an interactive session (qrsh) from the queue.
  • We shall use the GNU Screen utility so we can do other things while waiting.

GNU Screen

Simply allows several command-line sessions to which you switch back and forth. To enter a new session:

screen
  • This will open with quite bare screen except with a bottom line
  • there will be two command-line windows open.
  • Two switch back and forth, you use Ctrl+l,n (n for next) or Ctrl+l,p (p for previous)