Introduction

Software for RADseq

module load pyrad

will load the necessary software, mainly muscle and vsearch.

Guides

The go-to tutorial for this is at:

with the pyrad module loaded, the "pyrad "executable is immediately available on the command line
one of "-p", "-d", "-D" and "-n" options are essential.
"pyrad -n" generates a "params.txt" file which contains settings for the run. A large part of the analysis can be configured by editing this file.