MinION (Oxford Nanopore)

Introduction

Entirely unique sequencing method, where the flowcell is inserted into a USB container, and from there plugged into a computer.

Due to its small size in comparison with Illumina, IonTorrent and PacBio, this sequencing tool is eminently suited to field work.

Overview

The software required can be split into two groups of programs:

• Sequencing generation
  • MinKNOW, for control of MinION device & run parameters
  • Metrichor, for cloud basecalling of event data
  • MinoTour, live monitoring / control of run while sequencing
  • Chronolapse a screen image grabber for record keeping
  • TeamViewer, for remote control of MinION computer

• Sequence File Analysis
  • Poretools, poRe Sequence extraction and data summaries