Edgen RNAseq

Introduction

This is based on Edinburgh Genomics' two day RNAseq course. The protocol it follows is started to get dated, as certain elements are now moving to updated methods. Neverthelesss it reflects more or less the standard set of procedures that have been used for the four years of 2012-2015.

Steps

There are four essential steps 1) Mapping to reference 2) Gene count 3) Differential gene expression calculation 4) Functional analysis. An extra two quality control steps are also added to make 6 steps in total.