RNASeq library preparation
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Contents
Total RNA
mRNA-seq workflow
Illumina NGS Library
Illumina library read binding 1
Illumina library read binding 2
Illumina library read binding 3
RNA-Seq applications
- rRNA depleted samples
- Very high dynamic range
- No prior knowledge of expressed genes.
- - Gives more information than microarrays.
- Applications
- - Sequencing of mRNA
- - Differential expression of known or unknown transcripts during a treatment or condition
- - Isoform detection.
- - Alternative splicing events
- - Post-transcriptional mutations or editing
- - Gene fusions
- - Non-coding RNAs
- - miRNAs/small non-Coding RNA
NGS Data Analysis
