MinION Coverage sensitivity analysis

Subtract Operations

In the spirit of discovering new sequences, this ivolves subtract what is currently annotated from our alignment dataset.

This is a highly untargeted approach because all currently annotated genes and interestung areas are subtracted so that the coverage can only describe currently unknown areas of the genome, including non-coding areas.

Though this might sound unfruitful to start with, it has some benefits:

• useful to gain a feeling of general coverage
• reduces reliance on currently known areas, which may have been chosen becaus eof high coverage from previous studies.