Difference between revisions of "RNASeq library preparation"
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| + | = The number of replicates issue = | ||
| + | [[File:rnasoc.png]] | ||
| + | * http://rnajournal.cshlp.org/content/22/6/839.short | ||
| + | [[FIle:biorpaper.png]] | ||
| + | * http://www.biorxiv.org/content/early/2016/12/02/090753 | ||
Latest revision as of 10:27, 9 May 2017
Contents
Total RNA
mRNA-seq workflow
Illumina NGS Library
Illumina library fragment
Illumina library read bindings 1
Illumina library read bindings 2
RNA-Seq applications
- rRNA depleted samples
- Very high dynamic range
- No prior knowledge of expressed genes.
- - Gives more information than microarrays.
- Applications
- - Sequencing of mRNA
- - Differential expression of known or unknown transcripts during a treatment or condition
- - Isoform detection.
- - Alternative splicing events
- - Post-transcriptional mutations or editing
- - Gene fusions
- - Non-coding RNAs
- - miRNAs/small non-Coding RNA
NGS Data Analysis
The number of replicates issue
