Difference between revisions of "Pyrad"
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* The first stage is to gather statistics about your short read dataset and the barcodes. This is done via: | * The first stage is to gather statistics about your short read dataset and the barcodes. This is done via: | ||
pyRAD -p params.txt -s 1 | pyRAD -p params.txt -s 1 | ||
| − | * The second stage editing the raw reads and | + | * The second stage editing the raw reads and is done via: |
pyRAD -p params.txt -s 2 | pyRAD -p params.txt -s 2 | ||
Revision as of 17:04, 20 April 2016
Introduction
Software for RADseq
module load pyrad
will load the necessary software, mainly muscle and vsearch.
Guides
The go-to tutorial for this is at:
highlights
- with the pyrad module loaded, the "pyrad "executable is immediately available on the command line
- one of "-p", "-d", "-D" and "-n" options are essential.
- "pyrad -n" generates a "params.txt" file which contains settings for the run. A large part of the analysis can be configured by editing this file.
- The first stage is to gather statistics about your short read dataset and the barcodes. This is done via:
pyRAD -p params.txt -s 1
- The second stage editing the raw reads and is done via:
pyRAD -p params.txt -s 2
