RNASeq library preparation - Revision history

Rf at 10:27, 9 May 2017

2017-05-09T10:27:08Z

Rf at 09:32, 9 May 2017

2017-05-09T09:32:56Z

Rf: Created page with "= Total RNA = File:totalrna.png = mRNA-seq workflow = File:rnawf.png = Illumina NGS Library = File:illu.png = Illumina library read binding 1= File:illu2...."

2017-05-09T09:23:27Z

Created page with "= Total RNA = File:totalrna.png = mRNA-seq workflow = File:rnawf.png = Illumina NGS Library = File:illu.png = Illumina library read binding 1= File:illu2...."

New page

= Total RNA =

[[File:totalrna.png]]

= mRNA-seq workflow =

[[File:rnawf.png]]

= Illumina NGS Library =

[[File:illu.png]]

= Illumina library read binding 1=

[[File:illu2.png]]

= Illumina library read binding 2=

[[File:illu4.png]]

= Illumina library read binding 3=

[[File:illu5.png]]

= RNA-Seq applications =

* rRNA depleted samples
* Very high dynamic range
* No prior knowledge of expressed genes.
:- Gives more information than microarrays.
* Applications
:- Sequencing of mRNA
:- Differential expression of known or unknown transcripts during a treatment or condition
:- Isoform detection.
:- Alternative splicing events
:- Post-transcriptional mutations or editing
:- Gene fusions
:- Non-coding RNAs
:- miRNAs/small non-Coding RNA

= NGS Data Analysis =

[[file:dan.png]]

← Older revision		Revision as of 10:27, 9 May 2017
Line 42:		Line 42:

	[[file:dan.png]]		[[file:dan.png]]
		+
		+	= The number of replicates issue =
		+	[[File:rnasoc.png]]
		+	* http://rnajournal.cshlp.org/content/22/6/839.short
		+	[[FIle:biorpaper.png]]
		+	* http://www.biorxiv.org/content/early/2016/12/02/090753

@@ Line 11: / Line 11: @@
 [[File:illu.png]]
-= Illumina library read binding 1=
+= Illumina library fragment =
 [[File:illu2.png]]
-= Illumina library read binding 2=
+= Illumina library read bindings 1=
 [[File:illu4.png]]
-= Illumina library read binding 3=
+= Illumina library read bindings 2=
 [[File:illu5.png]]