Velvet

2016-04-07T09:11:25Z

138.251.175.12:

This is Daniel Zerbino and Ewan Birney's de-novo genome assembler and is one of the most widely used.

It consists of two stages, a hashing stage invoked by the '''velveth''' program, and then the actual genome assembly stage
which is invoked by the '''velvetg''' program.

As with many other genome assemblers, the program splits short reads into even shorter sequences of length k, called kmers and
arranges these in de Bruijn graphs from which the genome is then assembled.

== input fastq datasets ==

Velvet differs from other assemblers in requiring that the input short reads, if consisting of pair-end reads, be provided within a single file rather than the more usual two files (i.e. one file for each member of the pair). For Velvet, the two member-reads of each corresponding pair need to follow each other within a single file in what may be called an interleaved, or shuffled, format. The following diagram illustrates the procedure:

[[File:intleaved.png]]

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Velvet