Difference between revisions of "MinION (Oxford Nanopore)"
| Line 16: | Line 16: | ||
** '''MinKNOW''', for control of MinION device & run parameters | ** '''MinKNOW''', for control of MinION device & run parameters | ||
** '''Metrichor''', for cloud basecalling of event data | ** '''Metrichor''', for cloud basecalling of event data | ||
| − | |||
** '''Chronolapse''' a screen image grabber for record keeping | ** '''Chronolapse''' a screen image grabber for record keeping | ||
** '''TeamViewer''', for remote control of MinION computer | ** '''TeamViewer''', for remote control of MinION computer | ||
| + | ** '''MinoTour''', live monitoring / control of run while sequencing (a collaboration with Matt Loose of Nottingham University). | ||
* Sequence File Analysis | * Sequence File Analysis | ||
| − | ** '''Poretools''', poRe Sequence extraction and data summaries | + | ** '''Poretools''', poRe Sequence extraction and data summaries (deevloped by Nick Loman and Aaron Quinlan (latter of bedtools fame)). |
= Links = | = Links = | ||
* [http://www.nature.com/nature/journal/v530/n7589/abs/nature16996.html Nature paper 11 Feb 2016] describing Minion use in Ebola outbreak | * [http://www.nature.com/nature/journal/v530/n7589/abs/nature16996.html Nature paper 11 Feb 2016] describing Minion use in Ebola outbreak | ||
Revision as of 11:35, 2 February 2017
Introduction
Entirely unique sequencing method, where the flowcell is inserted into a USB container, and from there plugged into a computer.
Due to its small size in comparison with Illumina, IonTorrent and PacBio, this sequencing tool is eminently suited to field work.
Overview
Reputed advantages
- flowcell pores good for several runs, until they die out, which they may at different times.
The software required can be split into two groups of programs:
- Sequencing generation
- MinKNOW, for control of MinION device & run parameters
- Metrichor, for cloud basecalling of event data
- Chronolapse a screen image grabber for record keeping
- TeamViewer, for remote control of MinION computer
- MinoTour, live monitoring / control of run while sequencing (a collaboration with Matt Loose of Nottingham University).
- Sequence File Analysis
- Poretools, poRe Sequence extraction and data summaries (deevloped by Nick Loman and Aaron Quinlan (latter of bedtools fame)).
Links
- Nature paper 11 Feb 2016 describing Minion use in Ebola outbreak
