Difference between revisions of "MinION (Oxford Nanopore)"
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* Sequence File Analysis | * Sequence File Analysis | ||
** '''Poretools''', poRe Sequence extraction and data summaries | ** '''Poretools''', poRe Sequence extraction and data summaries | ||
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| + | = Links = | ||
| + | * [http://www.nature.com/nature/journal/v530/n7589/abs/nature16996.html Nature paper 11 Feb 2016] describing Minion use in Ebola outbreak | ||
Revision as of 11:32, 2 February 2017
Introduction
Entirely unique sequencing method, where the flowcell is inserted into a USB container, and from there plugged into a computer.
Due to its small size in comparison with Illumina, IonTorrent and PacBio, this sequencing tool is eminently suited to field work.
Overview
Reputed advantages
- flowcell pores good for several runs, until they die out, which they may at different times.
The software required can be split into two groups of programs:
- Sequencing generation
- MinKNOW, for control of MinION device & run parameters
- Metrichor, for cloud basecalling of event data
- MinoTour, live monitoring / control of run while sequencing
- Chronolapse a screen image grabber for record keeping
- TeamViewer, for remote control of MinION computer
- Sequence File Analysis
- Poretools, poRe Sequence extraction and data summaries
Links
- Nature paper 11 Feb 2016 describing Minion use in Ebola outbreak
