Difference between revisions of "MinION (Oxford Nanopore)"

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= Overview =
 
= Overview =
 +
 +
Reputed advantages
 +
* flowcell pores good for several runs, until they die out, which they may at different times.
 +
  
 
The software required can be split into two groups of programs:
 
The software required can be split into two groups of programs:
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* Sequence File Analysis
 
* Sequence File Analysis
** Poretools, poRe Sequence extraction and data summaries
+
** '''Poretools''', poRe Sequence extraction and data summaries

Revision as of 16:29, 1 February 2017

Introduction

Entirely unique sequencing method, where the flowcell is inserted into a USB container, and from there plugged into a computer.

Due to its small size in comparison with Illumina, IonTorrent and PacBio, this sequencing tool is eminently suited to field work.

Overview

Reputed advantages

  • flowcell pores good for several runs, until they die out, which they may at different times.


The software required can be split into two groups of programs:

  • Sequencing generation
    • MinKNOW, for control of MinION device & run parameters
    • Metrichor, for cloud basecalling of event data
    • MinoTour, live monitoring / control of run while sequencing
    • Chronolapse a screen image grabber for record keeping
    • TeamViewer, for remote control of MinION computer
  • Sequence File Analysis
    • Poretools, poRe Sequence extraction and data summaries