Difference between revisions of "Edgen RNAseq"
(Created page with "= Introduction = This is based on Edinburgh Genomics' two day RNAseq course. = Steps in Brief =") |
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= Introduction = | = Introduction = | ||
| − | This is based on Edinburgh Genomics' two day RNAseq course. | + | This is based on Edinburgh Genomics' two day RNAseq course. The protocol it follows is started to get dated, as certain elements are now moving to updated methods. Neverthelesss it reflects more or less the standard set of procedures that have been used for the four years of 2012-2015. |
| − | = Steps in | + | = Steps = |
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| + | There are four essential steps 1) Mapping to reference 2) Gene count 3) Differential gene expression calculation 4) Functional analysis. An extra two quality control steps are also added to make 6 steps in total. | ||
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| + | == Dataset preprocessing and quality control == | ||
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| + | == Mapping to a reference genome == | ||
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| + | == Quality Control of the mapping == | ||
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| + | == Estimating gene count == | ||
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| + | == Differential gene expression == | ||
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| + | == Functional Analysis == | ||
Revision as of 22:08, 25 May 2016
Contents
Introduction
This is based on Edinburgh Genomics' two day RNAseq course. The protocol it follows is started to get dated, as certain elements are now moving to updated methods. Neverthelesss it reflects more or less the standard set of procedures that have been used for the four years of 2012-2015.
Steps
There are four essential steps 1) Mapping to reference 2) Gene count 3) Differential gene expression calculation 4) Functional analysis. An extra two quality control steps are also added to make 6 steps in total.
