Difference between revisions of "Bowtie"

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(Created page with "= Introduction = This article refers to the early version of bowtie, which in fact has a few features that version 2 does not have, such as colorspace data processing = Inde...")
 
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  bowtie-build NC_002127.fna e_coli_O157_H7
 
  bowtie-build NC_002127.fna e_coli_O157_H7
  
Output files will have the extension '''ebwt'''.
+
<ins>Explanation</ins>:
 +
* '''bowtie-build''' is indexing creation tol of bowtie
 +
* in this example '''NC_002127.fna''' is our reference and is the first argument
 +
* the second argument is a label for the output index files, in this case '''e_coli_O157_H7'''
 +
* The extensions of the output files will have the extension '''ebwt'''. The root name will be the label mentioned above with some changing added characters.

Revision as of 13:02, 15 November 2016

Introduction

This article refers to the early version of bowtie, which in fact has a few features that version 2 does not have, such as colorspace data processing

Indexing the reference

This is done using the bowtie-build command and the input reference with the second argument being a label for the output files. It is an easy command, no options are required. Here is an example:

bowtie-build NC_002127.fna e_coli_O157_H7

Explanation:

  • bowtie-build is indexing creation tol of bowtie
  • in this example NC_002127.fna is our reference and is the first argument
  • the second argument is a label for the output index files, in this case e_coli_O157_H7
  • The extensions of the output files will have the extension ebwt. The root name will be the label mentioned above with some changing added characters.