FastQCFastQC Report
Wed 3 Aug 2016
MR236109Y_S9_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR236109Y_S9_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960218
Sequences flagged as poor quality0
Sequence length32-251
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTAT1957.035851E-964.555885245
TAAGGTT600.00636121553.6916545
TATAGAA1100.00141342839.0484772
ACCCTAA1150.001758405937.3507161
TTCTAGA1300.003208532533.041022
GGGTTTT26350.030.029247245
TTAGTCA1550.007583181427.7118244
TCCAATA1956.980205E-427.5341822
TTTGGGG37850.026.131794245
GGGGGGG2077850.025.01244245
CAATACA2150.001234195924.9728644
TTTTTTT46950.023.365046245
CTTGTAT2300.001827854923.3441983
ATAAGGC2300.001827854923.3441983
GGTTTTT17750.023.302465245
CTTTTTT15700.022.908823245
GTAATAC2350.002071361522.8475113
GTTTTTT19250.022.420893245
GTGTAAT2400.002340940322.3715231
TTTTTTG9000.021.97974245