FastQCFastQC Report
Wed 3 Aug 2016
MR236109Y_S9_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR236109Y_S9_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960218
Sequences flagged as poor quality0
Sequence length32-251
%GC63

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTT4700.094.47735245
GCTATAA358.61369E-488.902383
TATACAA1250.001594294976.12174245
TGTAATA901.1149314E-769.146292
GTATAGA2304.7873982E-836.076331
CTTAGTC1300.003803179431.9136733
GTTAGAC1350.004574672330.7316843
ATACGAT2053.219535E-530.356913
TCGTATA1500.007654767527.6585182
TATACGA1500.007654767527.6585182
CATATAC1550.0089824826.7663063
TCTCCTT8101.8368874E-423.494364245
GTGTAGA6709.094947E-1221.672721
TTCATAG2450.00322750521.1672332
GTATACG3004.2725858E-420.7438891
CTGTAGA3308.118941E-418.8580822
TCTTGTA3851.0522863E-418.858082
TCTACAC5502.349243E-718.858083
GTAATAC2850.00773846118.1963923
GTTCATA2850.00773846118.1963921