FastQCFastQC Report
Wed 3 Aug 2016
MR234466J_S7_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR234466J_S7_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490317
Sequences flagged as poor quality0
Sequence length32-251
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTAT2953.8198777E-1156.862724245
CTATTAG650.00957522348.4159661
TTTTATT1800.001057804441.418526245
TTAGTCT1050.001261711839.9623834
TAATAAG1150.001972704736.4873922
CTTTATT1703.178102E-732.891182245
GGTTTTT29050.032.07975245
ATTAGGA1653.0104464E-431.788262
GTCTTAG2002.5182066E-531.4703771
TTTTTTT70450.029.366293245
TAAAAAA2550.00328575929.236605245
CCTTAGA1855.884145E-428.351692
TATTACA1500.007244239627.9736672
ATCTAGA1906.8771146E-427.6055951
GGGGGGG3060550.026.064623245
GTTTTTT32650.025.117514245
TTAGGAC2150.001414740224.3956413
TCTAGGT2200.001617390323.8411942
GTCTAGG4052.3366374E-723.311391
TCTAGGA2250.001843345123.311392