FastQCFastQC Report
Wed 3 Aug 2016
MR234466J_S7_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR234466J_S7_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490317
Sequences flagged as poor quality0
Sequence length32-251
%GC63

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTT6700.0141.6431245
GTATAAG1050.001514166738.5066263
TAATACT1250.003561533532.3455664
CTATAAC1250.003561533532.3455662
GTCTAGA2453.900179E-628.879971
GTGTAAG2104.52636E-528.879971
CTTTGTA2904.589583E-727.8841082
CCTATAA1450.007358519427.8841081
TTCTAAT1500.00868206426.954642
TAAGACA1908.5420447E-426.599974
ACTAAGC2050.001330637624.6536353
TAGACAG2901.4448067E-524.3985945
TCTAATG2100.00153115224.0666433
TGCTAGA2150.001755898523.5069522
TGTAATG2150.001755898523.5069522
TAACAGT2200.002007108222.9727044
CTAGGGT2200.002007108222.9727044
TAATGCT2702.496228E-422.46224
TGTAACG3703.8297785E-621.8551122
GTGTAGG10000.021.2267781