FastQCFastQC Report
Thu 13 Apr 2017
MR405668F_S5_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR405668F_S5_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843541
Sequences flagged as poor quality0
Sequence length32-241
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA9300.024.7035081
GTATAGA9700.023.6848051
ATACTAG2900.00474339919.8053971
GTATACG4951.567711E-518.565061
GTATTAG11300.018.2980841
CTATTAG7501.12073394E-716.8477941
AGACTGT8201.6896593E-816.8104366
GTATATG10255.4569682E-1116.8104361
GTATAAG9805.18412E-1016.4101871
CCCTTGG4953.291997E-416.2444291
TAGACTG8552.8285285E-816.1222885
TAATACA19450.015.9461474
GTGTTGT7301.5623154E-615.7357961
ATACCGT8904.6326022E-815.4882676
TATACAC6701.2092187E-515.4304753
GTATAAT9809.140422E-915.238031
ACAGTGC10651.6825652E-915.1004548
GTATTAA26350.014.8221051
TTAGACT8655.6625686E-714.6079134
TATTAGG10251.6525519E-814.5690442