FastQCFastQC Report
Thu 13 Apr 2017
MR405668F_S5_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR405668F_S5_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843541
Sequences flagged as poor quality0
Sequence length32-241
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA10350.023.3063811
GTATACG4203.7897516E-621.879461
TACACAG11100.019.6619475
GTATATG9901.8189894E-1218.5643881
TAGGGAG3100.006974515518.5269625
CTAGGCC3100.006974515518.5269623
GTCTATG8751.03682396E-1018.3787461
GTGTATG6353.63636E-718.0893171
CTAATAC11050.017.6718713
GTATGTG5903.6123438E-617.5221081
AGAGTCC3950.001367578317.4481758
CTATAGT6605.4514567E-717.4041164
GTGTATA5453.585832E-516.8612351
TATAGTA7501.1211887E-716.8471832
CTGGGAC4250.00223164316.216543
CTATACC12101.8189894E-1216.1383614
CCTAAGA4350.002606380715.8437462
TGGGGTG5806.109765E-515.8437465
ACCGCCC5104.133189E-415.7660818
GTATAAA23900.015.3797041