FastQCFastQC Report
Tue 25 Apr 2017
MR393350T_S8_L001_R2_001_r79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR393350T_S8_L001_R2_001_r79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195083
Sequences flagged as poor quality0
Sequence length32-241
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG2056.6678424E-427.7513622
TTAGACC2056.6678424E-427.7513624
TAGGCTC2056.6678424E-427.7513625
GTATAAG6252.5465852E-1123.6732961
CCTAAGG3401.979484E-523.4254133
GTATAGA5453.1886884E-922.9716191
TTGGACC3203.5362932E-421.333864
GTATATA6459.640644E-1021.1746851
GTATAGG4305.0361596E-621.1746851
TCGGGGG6305.1725405E-520.68242235
GTATATG8301.8189894E-1220.568781
CTTACAC6151.2891178E-820.3513
TATACTG8950.020.3406625
GTCCTAA2800.004085422520.3239151
GTACTAT4507.4637137E-620.2335851
TAGACCG3557.1196724E-419.230525
GTGTAAG6552.6497219E-819.1137891
TATTAGA4801.3063247E-518.9634282
GTATAAA15150.018.7811761
TAGGGGG3150.00808156418.060415