FastQCFastQC Report
Tue 25 Apr 2017
MR393350T_S8_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR393350T_S8_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461428
Sequences flagged as poor quality0
Sequence length32-241
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA2304.014658E-529.386074
TATACTG3857.167182E-826.332975
GTATAGG2250.001216174125.0325781
TAGACAG2400.001771058123.4680425
ACCTAAT3502.6714551E-522.529321
GTGTGAG2600.00281985721.6628071
GTATAGC3153.397602E-421.4564951
GTGTATG3203.7789386E-421.1212371
CCTTATA3754.549089E-521.0273671
GTATATA4856.6565553E-720.9034921
TGTAAGG3855.5712073E-520.4811972
CTACACC5059.812229E-720.075634
AAGGAAG4650.004445018220.023342235
GTATAAG3405.6879816E-419.8788131
TGTAACA5201.299135E-619.4965272
GCTCTAC2950.005858845519.0926441
GTGTAAG3000.006456008618.7744331
CTACATG3608.3562435E-418.7744334
TATACAG5552.4229357E-618.2670155
GCTATAG3100.00780046618.1688061