FastQCFastQC Report
Tue 25 Apr 2017
MR393350T_S8_L001_R2_001_concat.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR393350T_S8_L001_R2_001_concat.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656511
Sequences flagged as poor quality0
Sequence length32-241
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA11150.023.4111041
GTATAGG6201.4577381E-820.135851
TAGGATG4007.079621E-519.858275
TTATACT12150.018.679154
GTCCAAG4301.2320101E-418.47561
CTTCCTA3200.00898059317.7332741
TAGTAGT6404.4930857E-717.7305984
GTATAGC11550.017.6872161
CCCTATA6454.875001E-717.5931512
TAGGCTC3250.00982769317.4578195
GTATACG7258.7520675E-817.2196221
GTCCTAA4652.2420303E-417.0849631
GTATAAA24700.017.000971
TATACTG12400.016.47235
CTTATAC10408.185452E-1116.3667073
TCGTATA6257.0292153E-616.340522
GTATATG14000.016.2132821
TAGACTG4903.346766E-416.2108355
GTATTAA29150.015.962991
GTACTAA6408.792042E-615.9599481