FastQCFastQC Report
Tue 25 Apr 2017
MR393350T_S8_L001_R1_001_r79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR393350T_S8_L001_R1_001_r79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195083
Sequences flagged as poor quality0
Sequence length32-241
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA7350.027.860441
GTGTAGA6951.07320375E-1021.2794971
ATAGGGC3905.7127276E-520.4190834
TATATAC6701.5588739E-920.3755463
CTACACA5109.837786E-720.0759074
TAGAGGG3455.8797613E-419.784954
CTAAGGT4301.2093746E-418.5196344
GTACATA6804.091271E-818.4029141
GTGTATG7851.1126758E-817.3905941
ATATACC9903.6379788E-1117.2368894
GTATAAC9903.6379788E-1117.2368891
ATATAAT11501.8189894E-1216.8172071
GTGTATA5453.897459E-516.6992231
TAGGCTG4802.804526E-416.5905065
TAAGACC5554.554395E-516.3983384
TATATGA9103.9035513E-916.2519252
TCTACAC7701.6906415E-716.2519253
TATGTAG4250.002381014916.0607242
GTGTTAA15300.015.61459351
TATGACC14600.015.5840354