FastQCFastQC Report
Tue 25 Apr 2017
MR393350T_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR393350T_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461428
Sequences flagged as poor quality0
Sequence length32-241
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAGA1350.00305881633.3611762
GTATAGA2851.5282785E-731.6053281
GTTCTAA2202.9734463E-530.7074471
CTCCTAA1853.8840657E-430.4308051
TAGAGCT1550.00601364329.0565094
GGTCTAC1550.00601364329.0565091
CATATAC3553.2769094E-828.5449523
TACTATA2250.001219491625.0208822
TATAGAG4101.3237695E-724.715752
ACTGTGC3651.3291192E-624.6781318
CCTTATA3002.4505166E-422.5187952
TCTATAC2550.00252610222.077253
ATAGACC2650.00315787421.2441468
GTATAAT4305.516644E-620.9477161
AGGACTT2800.004344436320.1060685
ATCCTAA3456.2954647E-419.581561
GTCATAT5902.0671541E-719.0837251
TATACAC2950.00587468119.0837253
TATACAG4951.859724E-518.1970065
TTATAGA4401.5584183E-417.9126784