FastQCFastQC Report
Tue 25 Apr 2017
MR393350T_S8_L001_R1_001_concat.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR393350T_S8_L001_R1_001_concat.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656511
Sequences flagged as poor quality0
Sequence length32-241
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA2056.7872327E-427.668024
GTATAGA9100.023.685041
TTCTAGA2400.001702137223.6330992
CATAGGA4553.3767174E-722.438462
TCTATAC7352.3464963E-1020.0640183
CTATACT6002.2892527E-718.906484
ACAGTCC7855.675247E-1018.7860578
TATAGTG10450.018.4541725
CCTTATA9251.4551915E-1118.3954932
GTATTAT21250.018.150221
TCTATAG5052.0765976E-517.9705163
ATAGGAC3250.00984613417.4521333
TAAGGAG11850.017.2312225
CCTATAC6606.2235995E-717.1877083
TATACAC10004.5474735E-1117.0158333
TATAAGG10757.2759576E-1216.8839262
CTATAGT6055.1752777E-616.8752044
GTGTATA7551.3970748E-716.5275171
TATAGAG11701.8189894E-1216.4825742
GTATAGG6459.508667E-615.8286811