FastQCFastQC Report
Thu 13 Apr 2017
MR334292Z_S9_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR334292Z_S9_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803246
Sequences flagged as poor quality0
Sequence length32-241
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGTG5051.8189894E-1229.5338949
TACCGTG9800.024.5845327
TATACCG15600.023.533915
ACCGTGT12400.021.2801598
GTATACG13650.019.3314271
ATACCGT17900.019.2281256
GTATAAG35800.018.266721
CTAGAGG8208.749339E-1018.1885553
TATACTG29850.018.0643465
CTCGGAC3850.001161675817.8796623
CGGACAG3250.00922637817.6504335
TTATACC38350.017.0521134
GTATAGT23200.016.8135591
GTATATA46250.016.620031
TGGGGGC4150.001920043316.5871544
GTATAGG19950.016.102151
CCTGTCT4400.002837319615.6447018
TAGACAG8102.7334136E-715.580325
ACAGACC8203.1417585E-715.3903168
TAGGGGT6801.4096449E-515.1845654