FastQCFastQC Report
Thu 13 Apr 2017
MR334292Z_S9_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMR334292Z_S9_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803246
Sequences flagged as poor quality0
Sequence length32-241
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGTG4650.032.0709539
CCAGTGC3452.0809695E-523.2755768
CCGTGCA3002.1630089E-422.9430669
TACCGTG9350.020.8573347
TACTCCG3100.00703097218.5024747
TATACCG15500.017.7623755
GTACTAG9152.0008883E-1017.5520741
CCCTGTG5302.8573213E-517.3155218
ACAGAGC6858.1691906E-716.7467638
ACGGTGC6256.3402676E-616.5190098
GTATATA48000.016.4903281
GACAGCG7001.0244203E-616.3879057
TATACTG30300.016.2797345
ACCGTGC5003.5937078E-416.0601468
GGTGTCC5053.876501E-415.9011368
GTCTATA21300.015.618521
ACAGTCC4450.003061452615.46723658
GTATATG38000.015.3960051
TAGGGTG8253.3701508E-715.2953785
CTATACT23350.015.2298734