FastQCFastQC Report
Thu 13 Apr 2017
397563R_S3_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename397563R_S3_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982265
Sequences flagged as poor quality0
Sequence length32-241
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG5403.6379788E-1227.579735
ACCGCGC2608.26E-526.4373748
GCTAGAC3902.038385E-623.4998873
CTAGACC4805.13568E-721.4803664
TAGGCCG2750.003543013720.8294475
TACCGCG2800.003932854620.457497
GTGTTAG8957.2759576E-1219.2003271
GTATTAG19600.018.7039931
GGCCGTG3759.826577E-418.3299147
AGACCGT6253.1666605E-718.3299146
GTATATA18300.018.1546271
TCTAGAC5853.4174882E-617.6249163
GACCGTG5252.6623853E-517.457067
TATAAGG13350.017.162842
GTATAGG8701.9572326E-917.1184541
TATAGGG4702.2635738E-417.062422
TATACTG11501.8189894E-1216.9352465
ACACCGC5453.6695677E-516.8164356
GTATAAG15100.016.6911451
AAGACCG6256.4154483E-616.4969225