FastQCFastQC Report
Thu 13 Apr 2017
397563R_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename397563R_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982265
Sequences flagged as poor quality0
Sequence length32-241
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGCG2550.002288278422.4593759
GTCTTAG7409.094947E-1221.670261
CTATACT10700.020.3393784
CTAAGAC5851.5821388E-719.579973
CTTAGAC4751.1263879E-519.291423
GAGTAGG4209.5792246E-519.090471
AGGACTG3050.006454974418.777515
GTCTAGA5602.2557979E-618.4086671
ACTGCGC4401.3685117E-418.2227218
ATACGGG4401.3685117E-418.2227216
CCTAGAC3800.001075240318.0857073
CTATTAG12100.017.9860611
TGGACCG3900.001279887917.6219735
GTAAGGG3900.001279887917.6219733
GTATAAG12500.017.4105071
TAAGACT11200.017.3859634
GCACCGT6004.3571854E-617.1814236
GTATTAG20750.017.112421
AGCACCG6757.107592E-716.9693055
TAAGGCG4752.457189E-416.8799925