FastQCFastQC Report
Thu 13 Apr 2017
396779D_S15_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename396779D_S15_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906181
Sequences flagged as poor quality0
Sequence length32-241
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAT1904.7087297E-429.4505651
GTTTTAT2057.3440105E-427.2956491
ATATAGT1700.00972738826.3322731
GGATATA2952.2811466E-422.7617951
GTACAGG7151.9463187E-1020.3476641
TAGAGGT4457.835726E-620.119044
GTCTTAT3957.145994E-519.8325351
TGTAGAA4559.494319E-619.6768632
TCTAGTG2950.00608887118.9681632
GTGCATT3659.589334E-418.3965191
ATAAGAT3150.00888895417.7638343
GGTTACA3800.001256481617.670341
TTCATAA3200.00973269617.4862752
ACTACAC7901.4724719E-816.9993153
CCTCTAG4652.4939005E-416.846991
TAGTGCT4702.7059673E-416.6677684
CTTAACG5306.747834E-414.7808514
GAATACT5407.7746063E-414.5071321
CCCTACG9400.004689032714.503535235
GTTCATA5508.9330814E-414.2433651