FastQCFastQC Report
Thu 13 Apr 2017
396779D_S15_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename396779D_S15_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906181
Sequences flagged as poor quality0
Sequence length32-241
%GC60

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATA1254.174299E-544.4658972
ATATAAG1550.00640409828.6876751
TAACACT2300.001494316424.1662464
TCTAGGA3251.6708267E-523.9431742
GTTCTAT2350.001693616923.6520711
CTAGACA2902.1261215E-422.9996014
GTATTAG2500.002426870422.2329481
CTACACA3052.9908505E-421.8684734
TACAGTG2550.002722581221.7970072
GGATAAA3103.3383028E-421.5157571
ATACCTA3103.3383028E-421.5157575
TATACCG2700.003792473320.5860615
TACAGGA4356.8217396E-620.444092
CTAGGAC4101.0017084E-418.9793453
CTAAGGT3609.143217E-418.5274544
ATAACAC3609.143217E-418.5274543
GTATACC3050.00767145218.2237283
CAATAGG3900.001563980417.1022681
GTACAGG7201.02299964E-716.9835011
GTCCTAG4903.9119908E-415.8806761