FastQCFastQC Report
Thu 13 Apr 2017
367980G_S7_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename367980G_S7_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187167
Sequences flagged as poor quality0
Sequence length32-241
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATA751.5045477E-461.501672
GTATTAG1203.9187944E-757.6578141
ACTTAAT650.006586239753.2225951
GTGTATA1707.9438905E-847.482911
ATAAAGT1006.226865E-446.126256
ACTAAGC1250.001866351536.9013
CTAGTGG1300.002262535735.481733
TGCTAGG2202.529104E-531.4497172
CTTATAT1853.3798334E-431.1663863
ATATAAC1500.00456118430.7508351
CTATGCT2303.4262375E-530.0823364
GTATACA2455.2717864E-528.2405621
ATATATC2056.165286E-428.1257633
TTATGAC2506.0496895E-527.6757533
TATCAAG3405.824659E-727.1330912
TACTGAG1700.00840642527.1330917
TATATAT2250.001061921425.6256962
GTGTAGG9500.025.4908261
ATGTAAC2400.001546825424.024093
GTATCAA3401.7845088E-523.7414551