FastQCFastQC Report
Thu 13 Apr 2017
367980G_S7_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename367980G_S7_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187167
Sequences flagged as poor quality0
Sequence length32-241
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATG450.001536721676.843834
CTATTAT956.642067E-660.6661761
GTCTAGT600.004806583357.632871
TATTATC852.7989165E-454.24272
TCTAGTT1304.2495318E-544.3329772
TTGTATA1109.977615E-441.9148141
TTATCCT1651.7310519E-434.9290164
TAAGGAC1651.7310519E-434.9290164
ATCCTAG1350.00272837634.1528131
GTACATA1350.00272837634.1528131
GTATAGG5051.8189894E-1229.6723691
TATAGGG3502.2793756E-829.6397632
GTATTTA1600.006266306628.8164351
CTATAGG1600.006266306628.8164351
ATATAGG2803.966734E-628.8164351
GTAGACT1650.00728227627.9432134
CCTATAG1650.00728227627.9432133
TAGCACA2506.067544E-527.6637784
TACAGAC2506.067544E-527.6637782
TAGTACT2107.1159954E-427.4442254