FastQCFastQC Report
Thu 13 Apr 2017
360761T_S14_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename360761T_S14_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196110
Sequences flagged as poor quality0
Sequence length32-241
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC2506.130589E-527.6218513
CCTAGAC3906.614755E-826.5594733
GTCTTAG3151.0024967E-525.5757871
CCTTAGT2851.4938976E-424.2296941
TAGACAG2851.4938976E-424.2296945
GTCTAGA3052.365528E-422.6408621
TAGACTA3102.6406866E-422.2756845
CTAGACT2600.00249174422.1328954
CTATGGT4855.430047E-721.3571014
CTCCTAG3253.6344325E-421.2475781
GTCCTGA9500.020.595241
CTAAGGC3404.928041E-420.3101843
AATACAG9850.019.8634285
GCTAGAC4107.675888E-519.649693
TATAGTG6502.1351298E-819.4769465
TAGGAGT5351.3926383E-619.361114
GTATTAG9550.019.282271
TTCTAGG4209.236615E-519.181842
GTGTATG8456.184564E-1119.0683381
TCCTAGA4251.01148755E-418.9561712