FastQCFastQC Report
Thu 13 Apr 2017
360761T_S14_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename360761T_S14_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196110
Sequences flagged as poor quality0
Sequence length32-241
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG3006.899123E-626.8369881
TACACTC4304.598154E-621.3982625
TGTAAGG3253.6505517E-421.233662
TATAGTG6008.56744E-921.0862055
TATACCC5007.3230876E-720.7028185
GTAGGGT3455.4617267E-420.0027224
GTGTAGA9800.019.9516941
TTAAGGG4107.714639E-519.636823
TATAAGG5451.6732956E-618.9934122
TACTAGG3100.00692437218.550912
TATACAC6202.793331E-718.550913
CTCTAGG3100.00692437218.550912
TAAGGGT5001.6920267E-518.4025044
TCTATAC7808.978532E-917.6947173
ATAAGAG5403.2786098E-517.0393563
GTCCTAT4752.3816597E-416.9496751
GTATAAG6857.943381E-716.7906071
GTATAAA13850.016.6087591
GTATATA9805.0931703E-1016.4308071
TAGACAG4250.002213198516.2375055