FastQCFastQC Report
Thu 13 Apr 2017
349032A_S13_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename349032A_S13_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588287
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG11850.018.8700355
TACCGTG8201.8189894E-1218.1796687
ACTGTCC5405.9778904E-717.2538538
GTATAGG12600.017.0121941
ACTGTGC8005.693437E-1016.304898
CCGTGCA6301.6728336E-716.2679169
CTGTCTT11956.6911176E-515.674952235
TCTATAC19100.015.6097673
TAAGCCG6602.8381874E-715.5284675
AGACTGC4305.551004E-415.1673396
GTATATA30550.014.9481661
CTATACT15650.014.8835154
TACACTG8151.274384E-814.86165
ACCGTGC8401.8975697E-814.4192918
ACCTAGG3950.005514668314.1565431
TATACTG19200.014.0726745
TCTAGAC5351.8283192E-413.9320833
TAGGACC8753.245077E-813.8425194
TATACTA24300.013.80308155
CATATAC25050.013.7617543