FastQCFastQC Report
Thu 13 Apr 2017
349032A_S13_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename349032A_S13_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588287
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACC4909.600626E-920.8801564
TAGACCG4108.3718624E-720.4171815
CCTATAC13850.019.4753533
TATACTG18850.017.7634895
CTATACT13950.017.3354954
TCTAGAC5958.892857E-817.1954213
GTCCTAT8254.9112714E-1116.91121
ACCGTGC7904.8748916E-1016.4830678
CTTAGGG3450.002270507616.175933
TATACCG10850.015.4304955
CCAAGCC4858.0490325E-515.3421188
ACTGATC11001.8189894E-1215.2200788
TAGACAG4959.579312E-515.0321785
GTATATA28750.014.8818551
ACTGCTC9554.5292836E-1014.6091528
AGACAGT10401.07320375E-1014.3094776
TATACAG19200.014.0486285
GTCCTAG5301.7129422E-414.0394861
GTGTATG13950.014.0017461
TACTGTG8652.8538125E-813.97862157