FastQCFastQC Report
Mon 10 Oct 2016
Undetermined_S0_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658827
Sequences flagged as poor quality0
Sequence length32-241
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC33720.5118187323834633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGT1805.9380836E-638.8549274
CTAGTAC2953.8508006E-935.564841
CTAATAC2655.950824E-835.192041
CTAGAAA3004.5383786E-934.9720881
AACCTTA1701.9297199E-434.286362
CTTAGTC1500.00432398631.08635
TAGAAAG3052.0449988E-730.576692
CTAGTAA1550.0050762130.0835191
CGCCTTA2404.2510077E-529.143412
GCCTTAG2404.2532098E-529.1411973
CGACTTA2607.33382E-526.9016062
TTAGTCT1750.00918091126.64546
AGTAAGT2350.00128445324.8010183
TAGGGGA2851.3694188E-424.5399534
CTAGGAC2901.5401428E-424.1186811
ATAGGGC2951.7285513E-423.7116898
CTAGAAC2800.003554358120.8167211
AGGAATA3707.98411E-418.9038335
TAAGGAG3100.00640681318.8007723
AGTCTCG4651.8238819E-417.550058