FastQCFastQC Report
Mon 10 Oct 2016
Undetermined_S0_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658827
Sequences flagged as poor quality0
Sequence length32-241
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG801.9720478E-458.2225841
GTATAGA1905.2750693E-1155.1582371
TAGGACA1400.003101050933.2700464
TACAGTG1600.005960382529.1112925
TAGGCCC3301.31206125E-524.7004894
ACAGAGC2851.378994E-424.514776
TAGTTCC2450.001647110823.7643185
GTCCAAT2450.001647110823.7643181
CAGAGGT3152.7168944E-422.180034
CCTTAAA2750.003222368221.1718482
AGTCCCA2800.00357714420.793785
GTACAAG2950.004838923519.736471
ACGTAGA2950.004838923519.736471
CACAGAG3050.00586725919.089375
ATTGGAT3150.00706837618.483363
ATGACTG3809.622159E-418.386085
GGACTGA3850.001050483418.1472996
AGAGGTG4100.001601535517.0407565
TCAAGAC5503.447887E-516.9374793
TACACCG10207.512426E-1015.982675