FastQCFastQC Report
Mon 10 Oct 2016
367980G_S15_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename367980G_S15_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163999
Sequences flagged as poor quality0
Sequence length32-241
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGAT1850.068.8726653
TATAAGT650.00647099753.46064
TATGATC1759.6342774E-846.332524
CTTACTC1355.158523E-542.9004863
TACAGAG1109.7400893E-442.1204762
TAAAGGA1406.3927546E-541.3683244
CACTAAA1150.001212077340.289152
ATAGTGA1806.205155E-638.610436
ATACAAT1300.002213523335.6404043
TCATATA1350.00266365934.320392
GTATAGA2602.1431006E-631.1853521
CTTTAAT1500.004462664530.8883481
GTACAGA5350.030.3109951
GTGTAGA7650.030.2826961
GTCCTAT3102.4967085E-729.891951
TAATACA1600.006118205428.9578272
ATATATA1600.006118205428.9578271
TATTACA2005.198442E-428.9578272
ACTGTAC1600.006118205428.9578278
TATGATG2106.915225E-427.5788824