FastQCFastQC Report
Mon 10 Oct 2016
367980G_S15_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename367980G_S15_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163999
Sequences flagged as poor quality0
Sequence length32-241
%GC66

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTAG600.004738512457.8408361
ATAGAGT1057.795952E-444.0692068
TTATTCT1602.786719E-643.3806234
TATTACT1406.44167E-541.3148772
ATAAGAC1406.44167E-541.3148773
ATAGGAG1601.4142656E-436.150523
TATAGAA2256.994287E-735.9898532
TATATGC1300.00222760835.594362
GTATAGA3252.728484E-1035.594361
TATGTTG2001.2893061E-534.70455
CACTGTA1350.002680596234.2760472
TAGATTG1350.002680596234.2760475
TGTATAG1450.003804379131.9121842
GTATAGG4007.2759576E-1131.8124561
AAGTTAT1853.3175727E-431.2653147
AGGAGAG2252.8855919E-530.8484465
CCCTATA2252.8855919E-530.8484462
TCCTACT1500.004490977630.8484462
TAATGGT1500.004490977630.8484464
TAGACTG1550.00527210629.8533335