FastQCFastQC Report
Mon 10 Oct 2016
364588W_S11_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename364588W_S11_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079776
Sequences flagged as poor quality0
Sequence length32-241
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGTG1750.00983837826.2714945
TATATGG2600.002510943222.103425
GTCCTAT3653.1670046E-522.0428621
ATAGCAC4251.0217651E-418.930933
GTCTATG5602.183193E-618.4721431
CTTGTAT3200.00834754617.959033
TATTGAA3250.0091274317.6827372
TACTGGA9453.0559022E-1017.0278192
TCCTATG4150.001896051116.6175122
GTCCTAG4852.806207E-416.5889591
GTGTAGT6256.2199088E-616.5510431
GCTTGAA7950.002138506416.319096235
TCTACAC8052.4927795E-715.7057853
TATCCTG4550.003503029515.1566325
TACAGAC5355.919894E-415.0385895
CAATACT4650.004048074614.8306834
GTGTAGG8605.272577E-714.70134451
ACAGAGG6401.4073332E-414.3672243
GAACATA4800.0049984814.3672231
GTCCTAC7352.885789E-514.0740141