FastQCFastQC Report
Mon 10 Oct 2016
364588W_S11_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename364588W_S11_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079776
Sequences flagged as poor quality0
Sequence length32-241
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATTG1150.001274784139.87775
GTATAGA1806.6576595E-638.2161331
TAAGACT1702.1289372E-433.7201164
ATTAGAC1400.003347485332.7566833
ACCTAAT1450.003975074331.6271421
GATTAGA1450.003975074331.6271422
TTTAGGA1550.00550830229.5866812
CTCTAAG1550.00550830229.5866812
GTATACA2005.520009E-428.6620961
ATTAACC2107.342435E-427.2972343
TACAGGA4052.8114046E-622.6465972
GGATTAG3552.6057765E-522.6067261
ATAAGAC3102.7101938E-422.190013
AATACAT4057.194227E-519.8157735
GTATAGC2950.005296334619.431931
CTCTAGG2950.005296334619.431931
TATACGG4251.0417207E-418.8832652
TATTGAA3100.00705290318.4916762
ATAGGAC4351.2450948E-418.4491673
GTATTTG4702.2508333E-417.0752931