FastQCFastQC Report
Mon 10 Oct 2016
362711M_S7_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename362711M_S7_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762492
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAATG409.438652E-486.877312
TTAATGG1101.5368798E-552.6529163
ATATAAC903.621238E-451.4828531
TATTACA1151.9992518E-550.363662
TACATAC1857.483117E-637.568572
GTCATTA1908.98504E-636.579921
CGCCTAA2350.0020158936.320614235
GTATAAG1450.0037787531.954871
TATAACC1450.0037787531.954872
TACAAAA1853.2906118E-431.307142
TAGAACC3451.8868377E-830.2181954
TAATGGT1954.4797233E-429.7016454
GTTCTAG1600.006115583228.9591051
CTGTATT3150.008455940527.09633235
TATGAGA2157.929946E-426.93874
AAGAGAG2250.0010340325.7414255
AGAGAGA2709.899925E-525.7414256
TAACCCA3251.2142949E-524.9493834
TATTGAC3351.5356625E-524.2046242
GTGTAAC2400.001506275524.1325871